Hormones &
Metabolism Tests
Hormones and metabolism set the rhythm for your energy, mood, and long-term health.
When hormones are out of balance, you may notice fatigue, irregular cycles, fertility challenges, sleep problems, weight gain, or metabolic concerns such as thyroid dysfunction or insulin resistance. Functional hormone and metabolic testing provides clear insights into how your body regulates stress, reproduction, energy, and blood sugar control.
Testing is ordered through accredited Australian laboratories and interpreted alongside your health history for the most personalised care.
Unsure where to start, or facing unresolved health concerns?
Book a free 10-minute discovery call to help you decide your first steps, or book an Initial consultation to fully assess your health situation and start your supported health journey today.
Popular hormone test profiles
Complete Thyroid Profile
A blood panel that goes beyond TSH to assess Free T3, Free T4, Reverse T3, and thyroid antibodies for a complete thyroid picture.
DUTCH Cycle Mapping
Daily tracking of estrogen and progesterone across an entire menstrual cycle to reveal pattern-based imbalances affecting fertility or PMS.
DUTCH PLUS
A dried urine test measuring reproductive and adrenal hormones, plus their metabolites, alongside organic acids for neurotransmitters and nutritional status.
HuMAP + Comprehensive Neurotransmitters
A 24-hour urine profile reporting sex and adrenal hormones with metabolites, plus urinary neurotransmitters for mood/stress insight — a single, comprehensive combined test.
Complete Thyroid Profile
Assessing each step within thyroid function is important to ensure that the entire pathway is working effectively. Thyroid dysfunction is one of the most under-addressed conditions in healthcare with standard measures of thyroid function potentially missing proper evaluation.
The Complete Thyroid Profile goes far beyond a standard GP thyroid test, providing a detailed view of how your thyroid hormones are produced, converted, and utilised—so we can understand why symptoms are occurring, not just whether your results sit within a broad “normal” range.
What is Measured
TSH – Indicates how your brain is signalling your thyroid to make hormones.
Total T4 (Thyroxine): The amount of circulating hormone, although not all of this is available to the body to use.
Free T4: Thyroid hormone available for conversion. When required, it can enter your body’s cells and be converted into T3, the truly active form your body uses for energy and metabolism.
Total T3 & Free T3 – Active thyroid hormone that drives metabolism and energy.
Reverse T3 – Stress or illness may be converting thyroid hormone into its inactive form. Build-up of this can block healthy thyroid activity.
TPO Antibodies (TPO Abs) – Screens for immune activity that may affect thyroid tissue, such as in Hashimoto’s or Graves’ disease.
Thyroglobulin Antibodies (TG Abs) – Can help diagnose autoimmune thyroid disease or track thyroid health after cancer or surgery.
Who Benefits:
Consider this test if you experience:
Persistent fatigue or sluggish metabolism despite “normal” blood tests
Difficulty losing or maintaining weight
Hair thinning, dry skin, or feeling unusually cold
Anxiety, palpitations, or heat intolerance
Fertility or menstrual irregularities
Brain fog, poor concentration, or low mood
Family history of thyroid or autoimmune disease
Long-term stress, adrenal imbalance, or low iron
You are on thyroid medication: to evaluate conversion of inactive T4 into active T3, or check autoimmune activity, or if stress or nutrient issues are blocking T3 activity.
Why it’s Useful
Most thyroid screens measure only TSH (thyroid-stimulating hormone), which misses how much thyroid is available and its behavious in your body.
By evaluating all of these markers we can:
Identify under- or over-conversion issues between T4 and T3
Detect early thyroid autoimmunity or monitor efficacy of natural treatment approaches to reducing autoimmune activity
Optimise medication or natural thyroid support
Tailor nutrition for healthy hormone synthesis
Sample: Blood sample collected at your local pathology centre.
Turnaround: 3-4 weeks
Supplier: Precision Point Diagnostics (USA) via RN Labs, Australia.
Companion tests
For a broader view of how your hormones, stress, and neurotransmitters interact with thyroid function, this panel is often run alongside the DUTCH Plus or HuMAP + Comprehensive Neurotransmitters test.
These complementary profiles reveal how cortisol rhythm, sex-hormone balance, and nutrient metabolism influence thyroid performance—helping to build a full picture of hormonal health.
For deeper insight into your Hormones, book an Initial Consultation →
DUTCH Plus
Comprehensive Hormones + neurotransmitters & nutritional status
The DUTCH (Dried Urine Test for Comprehensive Hormones) Plus is the most comprehensive of the various 24-hour Dutch tests, providing an analysis of the major reproductive hormones, cortisol and related metabolites, alongside organic acids for neurotransmitters and nutritional status.
It allows for an integrated view of hormone production, metabolism, and stress response, and is particularly valuable for investigating fatigue, mood changes, hormonal imbalance, and sleep or stress-related concerns.
The Dutch Plus further includes salivary samples of morning cortisol to provide an accurate Cortisol Awakening Response (CAR) measurement. The combination with urinary cortisol and cortisone metabolites gives more in-depth insight into adrenal function to help analyse imbalances and guide towards more positive treatment outcomes.
What It Measures
Represents an entire day’s hormone production during peak Luteal phase
Sex Hormones & Metabolites: Of Oestrogens, Androgens, Progesterone, Cortisol, Melatonin, Adrenaline, Noradrenaline and 8-OHdG
Adrenal Hormones: Cortisol and cortisone patterns throughout the day, reflecting adrenal output and recovery capacity.
Cortisol Awakening Response (CAR): Three saliva samples taken within the first hour after waking assess HPA-axis responsiveness — a sensitive measure of stress adaptation.
Melatonin: Providing Insight your sleep-wake cycle and circadian rhythm, to provide insight into potential imbalances that affect sleep quality
Organic Acid Markers for neurotransmitters and nutrient metabolism to evaluate potential oxidative stress, nutritional deficiencies, gut dysbiosis, melatonin levels and neuroinflammation.
Metabolites of Oestrogens, Androgens, Progesterone, Cortisol, Melatonin, Adrenaline, Noradrenaline and 8-OHdG as well as organic acid markers for neurotransmitters and nutrient metabolism.
Who Benefits:
The Dutch Plus is suitable for both men and women to investigate:
Ongoing fatigue or burnout
Anxiety, low mood, or sleep disturbance
Low libido or fertility issues
Weight gain or metabolic sluggishness
Difficulty managing or recovering from chronic stress
For women: PMS, perimenopausal or menopausal symptoms, and HRT management post menopause.
Why It’s Useful:
The DUTCH Plus offers the most detailed picture of sex hormone metabolism and adrenal stress physiology in one integrated Dutch test.
It uses multiple sample types (urine and saliva) and adds Cortisol Awakening Response to provide a detailed look at cortisol patterns. This supports a more accurate understanding of whether symptoms stem from hormonal imbalance, poor hormone metabolism, or a disrupted stress response.
Sample: 4 dried urine samples and 5 saliva collections over 24 hours at home.
Turnaround: 3–4 weeks
Supplier: Precision Analytical (USA), via RN Labs, Australia
Looking for deeper insight into your Hormones? Book an Initial Consultation →
DUTCH Cycle Mapping
Dutch Cycle Mapping is a specialised hormone test that maps estrogen and progesterone patterns across an entire menstrual cycle.
Collecting daily samples over a month reveal how hormones rise and fall throughout the cycle. This provides a clear picture of whether ovulation is occurring, if progesterone production is adequate, and how estrogen and progesterone balance may be contributing to symptoms.
What it Measures:
Monthly pattern of estrogen (E1, E2) and progesterone (a- and ß-metabolites).
Who Benefits:
Women experiencing irregular or absent cycles, fertility challenges, PMS, perimenopausal transitions, or unexplained hormonal symptoms.
Why it’s Useful:
By seeing the full cycle, we can identify timing issues and imbalances that one-off tests often miss, helping to guide more targeted treatment.
The testing method is more accurate than saliva for progesterone and especially for oestradiol. The most accurate methods available are used for each test.
Sample Type: Frequent urine samples to be collected throughout the menstrual cycle.
Turnaround time: 3–4 weeks.
Supplier: Precision Analytical (USA) via RN Labs, Australia.
To discuss whether this test is suitable for you, book an Initial consultation →
HuMAP + Comprehensive Neurotransmitters
The most comprehensive test available for both hormone and neurotransmitter balance in a single profile.
HuMAP+ measures sex and adrenal hormones and their metabolites, along with key neurotransmitters such as serotonin, dopamine, GABA, noradrenaline, and adrenaline.
By assessing both hormone production and how hormones are metabolised, alongside brain chemical activity, the HuMAP+ test provides a unique window into the link between stress, mood, sleep, energy, and reproductive health.
What it Measures:
Sex hormones (oestrogen, progesterone, testosterone, DHEA)
Stress hormones (cortisol and cortisone)
Neurotransmitters (serotonin, dopamine, GABA, noradrenaline, and adrenaline)
How well your body processes and detoxifies its hormones (via hormone metabolites).
Who Benefits:
Women and men experiencing hormone changes (menopause, fatigue, PMS, low libido)
Those seeking answers to persistent symptoms
Anyone wanting to optimise long-term health and vitality.
Why it’s Useful:
HuMAP™ helps identify imbalances that may be causing fatigue, mood changes, sleep issues, or other wellbeing concerns.
It’s particularly useful when symptoms cross between hormone and nervous system function, for example:
anxiety with cycle changes
fatigue with stress, or
sleep disturbances with mood changes.
Sample: Urine
Turnaround: 3–4 weeks
Supplier: Doctors Data (USA), via RN Labs, Australia.
To discuss whether this test is suitable for you, book an Initial Consultation →
Hormone & Metabolism Testing – FAQs
-
Sometimes. Retesting a few months after intervention can help track progress and fine-tune your treatment plan.
-
Let your practitioner know, as some hormone medications can affect test results and interpretation.
-
Each sample type has its own advantages. Your practitioner will select the technology best suited to your personal health questions and medications.
-
For the DUTCH Complete, testing is usually done in the luteal phase for cycling women. DUTCH Cycle Mapping is collected across a full cycle. Thyroid and neurotransmitter tests can be done at any time.
-
Yes. Chronic stress influences cortisol, thyroid regulation, sex hormones, and even neurotransmitters. These tests show how your body is adapting so we can restore balance.
-
Yes. Hormones naturally fluctuate through life and may need to be monitored periodically during treatment or major life changes.
-
Male & Non-Cycling or Postmenopausal Female: Collect any day.
Cycling/Perimenopausal Female: For a 28-day cycle, begin collection between days 19 and 22. Collect 6-9 days before end of cycle.
Not sure when to collect? dutchtest.com/cycle-calculator
-
72 hours before collecting
Do not take Oral or sublingual DHEA, estrogen, or pregnenolone
Do not skip doses of birth control unless instructed by your medical provider
Consult your practitioner if taking: glucocorticoids: sublinguals, or oral hydrocortisone
Within 48 hours of samples
Food to Avoid: bananas, fava beans, creatine
Supplements to avoid: tyrosine L-dopa, D,L-phenylalanine (DLPA) mucuna, quercetin.
If taking hormones
Day time hormone doses: Collect samples midway between doses
Morning doses: Collect sample at least 2 hours after hormones
Night doses: Collect bedtime sample before evening hormones
Rules for collection day
Do not drink alcohol 24h before or on collection day
Do not drink more than 8oz of caffeine on collection day
Do not drink more than 40oz of fluid on collection day
Do not brush or floss teeth until after saliva samples in the morning or at bedtime
If urine is not returned within 48 hours, freeze samples.
Refer to your test kit for complete instructions.
-
5 days before and during test - Discontinue Cortisol/glucocorticoid supplementation:
Certain medications such as asthma inhalers and hydrocortisone contain cortisol. To evaluate natural cortisol production, it is recommended to stop cortisol containing products 5 days prior to sample collection.
Never discontinue prescription medications without first consulting your doctor.
3 days before and during test
If using hormonal supplementation:
Discontinue oral and sublingual hormones for 72 hours before and during test.
Do not use hormones vaginally, as this may directly contaminate the urine.
Other hormones such as topical creams and gels, injectables, and patches can be used on your regular schedule.
Do not skip doses of oral birth control pills unless instructed by your healthcare provider.
Note: A baseline hormone level may require supplement usage to stop for a longer period.
Call your health practitioner for more information.
24 hours before and during test - Avoid
alcohol, caffeine, tobacco or nicotine-containing products
strenuous exercise.
avocados, eggplant, tomatoes, bananas, melons, pineapple, grapefruit, plums, fruit juice, nuts, nut butters, wine, cheese, rice, and chocolate.
Day of Collection
Avoid all supplements and medications until after all samples have been collected
(including those that regulate allergy, mood, sleep, pain and inflammation).
Consult your practitioner for specific instructions before stopping any medications.
Never discontinue prescription medications without first consulting your doctor.
Urine samples collection
Samples should be frozen for at least 6 hours prior to posting.
Refer to your kit for full collection instructions.
-
This test is not meant for females not yet menstruating or males under the age of 12. To test children, salivary hormone testing may be a more appropriate medium.
-
Your collections should correspond to your pattern of eating and sleeping. The bedtime sample is collected before you go to sleep for your longest stretch of sleep and the waking is right after this sleeping period. The dinnertime
should be collected 4-7 hours before going to sleep.
Ready to understand how your hormones might be driving your health picture?
Book your free 10-minute discovery call to discuss how we might work together, or dive in fully with an initial consultation.
For more information about fees and packages, visit our Fees & Bookings page